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cardiovascular system
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Cardiothoracic Surgery
Drug modeling
Endoscopic class
Open abdominal class
Imaging services
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Surgical assistance
Functional laboratory
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Cell platform
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Molecular and protein platforms
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Functional laboratory
Animal platform
Cell platform
Biochemical testing platform
Pathology detection platform
Molecular and protein platforms
Behavioral platform
Instrument display (renamed)
Rat and rat treadmills
Ultrasound detection of cardiac function in rats and mice
Non-invasive blood pressure monitor
Live imaging of small animals
MRI detection
X-ray testing
ECG collection
Muscle tension detection
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Functional laboratory
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Molecular and protein platforms
免疫印迹(WB)
2024-04-16
蛋白质印迹法(免疫印迹试验)即Western Blot,其基本原理是通过特异性抗体对凝胶电泳处理过的细胞或生物组织样品进行着色。
酶联免疫吸附(ELISA)
2024-04-16
在固相载体(如聚苯乙烯反应板)上包被抗原或抗体后。通过抗原抗体反应使酶标抗体(或酶标抗原)结合到载体上。经洗涤使结合的酶标抗体和游离的酶标抗体分离,洗去游离的酶标抗体(或酶标抗原),加入底物显色。根据颜色深浅进行定性或定量分析。
蛋白质谱分析
2024-04-16
质谱法分析蛋白的基本原理是通过电离源将蛋白质分子转化为离子,然后利用质谱分析仪的电场、磁场将具有特定质量与电荷比值(M/Z)的蛋白质离子分离开来,经过离子检测器收集分离的离子,确定离子的M/Z值,分析鉴定未知蛋白。
免疫共沉淀(Co-IP)
2024-04-16
免疫共沉淀(Co-Immunoprecipitation)是以抗体和抗原之间的专一性作用为基础的用于研究蛋白质相互作用的经典方法。是确定两种蛋白质在完整细胞内生理性相互作用的有效方法。
载体构建
2024-04-15
载体构建(vectorconstruction),是把目的DNA分子运送到受体细胞中去,因此必须寻找一种能进入细胞、装载了外来的DNA片段后仍能照样复制的运载体。
定点突变
2024-04-15
定点突变是指通过聚合酶链式反应(PCR)等方法向目的DNA片段(可以是基因组,也可以是质粒)中引入所需变化(通常是表征有利方向的变化),包括碱基的添加、删除、点突变等。
甲基化检测
2024-04-15
DNA甲基化在DNA甲基化转移酶(DNMTs)的作用下的CpG二核苷酸5’端的胞嘧啶转变为5’-甲基胞嘧啶,是最早发现的修饰途径之一。
SNP
2024-04-15
SNP,即单核苷酸多态性(SingleNucleotide Polymorphism,SNP),主要是指在基因组水平上由单个核苷酸的变异所引起的DNA序列多态性。与其它分子标记相比,SNP 分辨率最高也最为丰富,覆盖基因组范围大,遗传上比较稳定。
siRNA
2024-04-15
siRNA是一种小RNA分子(~21-25核苷酸),由Dicer(RNAase Ⅲ家族中对双链RNA具有特异性的酶)加工而成。
miRNA
2024-04-15
小 RNA是 19~28 nt 的调控RNA分子,主要包括微 RNA(micro RNA,miRNA)和小干涉RNA(short interfering RNA,siRNA)两类。
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